High-purity circular RNA isolation method (RPAD) reveals vast collection of intronic circRNAs

High-purity circular RNA isolation method (RPAD) reveals vast collection of intronic circRNAs

High-throughput RNA sequencing methods coupled with specialized bioinformatic analyses have recently uncovered tens of thousands of unique circular (circ)RNAs, but their complete sequences, genes of origin and functions are largely unknown. Given that circRNAs lack free ends and are thus relatively stable, their association with microRNAs (miRNAs) and RNA-binding proteins (RBPs) can influence g...

Circular RNAs (circRNAs) in Health and Disease

Splicing events do not always produce a linear transcript. Circular RNAs (circRNAs) are a class of RNA that are emerging as key new members of the gene regulatory milieu, which are produced by back-splicing events within genes. In circRNA formation, rather than being spliced in a linear fashion, exons can be circularised by use of the 3' acceptor splice site of an upstream exon, leading to the ...

Protective Polymer Coatings for High-Throughput, High-Purity Cellular Isolation

Cell-based therapies are emerging as the next frontier of medicine, offering a plausible path forward in the treatment of many devastating diseases. Critically, current methods for antigen positive cell sorting lack a high throughput method for delivering ultrahigh purity populations, prohibiting the application of some cell-based therapies to widespread diseases. Here we show the first use of ...

RNA isolation (Total RNA Extraction from High Polysaccharide Plants). Fatemeh Mehrpooyan*

Isolation of Residual Kraft Lignin in High Yield and Purity

In this paper, we report on a novel residual lignin isolation method. Initially we demonstrate that, in order to cellulolytically isolate residual lignin in high yield, the cellulosic structure of the pulp should be exposed as much as possible. Consequently, we developed a two-step method that first uses a mild enzymatic treatment to render most of the carbohydrates water-soluble and remove hem...